Reporter
Part:BBa_J52028
Designed by: Jelka Pohar Group: iGEM06_Slovenia (2006-10-15)
green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag
GFP-linker-PEST191 is a part composed of GFP (as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence. Its estimated half-life is 2h.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 759
[edit]
Categories
Parameters
//function/reporter/fluorescence
abs | |
emit | 511? |
excite | 501? |
lum | |
protein | GFP-PEST |
tag | Other |