Reporter

Part:BBa_J52028

Designed by: Jelka Pohar   Group: iGEM06_Slovenia   (2006-10-15)

green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag

GFP-linker-PEST191 is a part composed of GFP (as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence. Its estimated half-life is 2h.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 759


[edit]
Categories
//function/reporter/fluorescence
Parameters
abs
emit511?
excite501?
lum
proteinGFP-PEST
tagOther